Related Experiment Video
Updated: Jun 25, 2026
An Intact Pericardium Ischemic Rodent Model
Published on: September 2, 2021
Effects of gamma irradiation on mechanical behavior and calcification of glutaraldehyde-fixed bovine pericardium
José Augusto Baucia1, Ricardo Mendes Leal Neto, José Roberto Rogero
1Instituto de Pesquisas Energéticas e Nucleares(IPEN) - Linked to the University of São Paulo, São Paulo, SP. jabaucia@hotmail.com
Objective:
To evaluate the effect of gamma irradiation on glutaraldehyde-fixed bovine pericardium.
Method:
Glutaraldehyde-fixed bovine pericardium was exposed to gamma radiation (doses from 0 to 10000 Gy). Six samples from each of nine groups were evaluated by optic microscopy, and shrinking and mechanical tests and the denaturation temperature was determined. Additionally, they were subcutaneously implanted in rats and after four months they were explanted and Ca2+ levels measured by atomic absorption spectroscopy.
Results:
The Ca2+ levels were (in microg/mg): control (0 Gy) - 194.45; 50 Gy - 154.64; 100 Gy - 169.37; 200 Gy - 163.64; 500 Gy - 199.89; 1000 Gy - 184.02; 2000 Gy - 198.95; 5000 Gy - 227.95; 10000 Gy - 362.62. Gamma irradiation caused a significant effect on the biomechanical properties of the tissue.
Conclusion:
e-fixed bovine pericardium.
