Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

Impedances and Admittance

Impedances and Admittance

In the realm of AC circuits, passive circuit elements like resistors, inductors, and capacitors take on a different character when characterized by phasor voltage and current. Their behavior is expressed through impedance, a vital concept in AC circuit analysis.
Impedance is a measure of resistance to sinusoidal current flow in an AC circuit. Unlike their behavior in DC circuits, where inductors appear as short circuits and capacitors as open circuits, the behavior of these components in AC...

Amperometry: Overview

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Qualitative and quantitative protease activity tests based on protein degradation in three-dimensional structures.

Bioelectrochemistry (Amsterdam, Netherlands)·2024

Same author

Quantitative determination of dopamine in the presence of interfering substances supported by machine learning tools.

Bioelectrochemistry (Amsterdam, Netherlands)·2024

Same author

Azimuthal Correlations within Exclusive Dijets with Large Momentum Transfer in Photon-Lead Collisions.

Physical review letters·2023

Same author

Photoelectrochemistry of a photosystem I - Ferredoxin construct on ITO electrodes.

Bioelectrochemistry (Amsterdam, Netherlands)·2023

Same author

Dyspepsia with Salty Taste.

The Homoeopathic physician·2023

Same author

Measurements of the associated production of a W boson and a charm quark in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2022

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Measuring Changes in Brain Endothelial Barrier Integrity with Two Impedance-based Biosensors in Response to Cancer Cells and Cytokines

Measuring Changes in Brain Endothelial Barrier Integrity with Two Impedance-based Biosensors in Response to Cancer Cells and Cytokines

Published on: September 22, 2023

Impedance spectroscopy and biosensing.

O Pänke¹, T Balkenhohl, J Kafka

¹Biosystems Technology, Wildau University of Applied Sciences, Bahnhofstrasse 1, 15745 Wildau, Germany.

Advances in Biochemical Engineering/Biotechnology

|November 10, 2007

Summary

This chapter details impedance measurement techniques for analytical applications. It covers biosensor designs using enzymes, antibodies, DNA, and cells, enhancing sensitivity for label-free DNA and autoantibody detection in celiac disease.

More Related Videos

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Published on: October 10, 2018

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Related Experiment Videos

Last Updated: Jul 10, 2026

Measuring Changes in Brain Endothelial Barrier Integrity with Two Impedance-based Biosensors in Response to Cancer Cells and Cytokines

Measuring Changes in Brain Endothelial Barrier Integrity with Two Impedance-based Biosensors in Response to Cancer Cells and Cytokines

Published on: September 22, 2023

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Published on: October 10, 2018

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Area of Science:

Electrochemistry
Biosensors
Analytical Chemistry

Background:

Impedance measurement is a versatile transduction technique.
Biosensors require sensitive and specific detection methods.

Purpose of the Study:

To introduce impedance measurement principles and applications in analytical chemistry.
To highlight advancements in electrode design and amplification for enhanced sensitivity.
To showcase label-free and immunoassay applications for disease diagnostics.

Main Methods:

Overview of impedance measurement techniques.
Description of electrode designs and amplification schemes.
Case studies on enzyme, antibody, DNA, and cell analysis.

Main Results:

Demonstration of label-free DNA determination using impedance.
Successful sensorial detection of celiac disease-associated autoantibodies.
Enhanced sensitivity achieved through optimized electrode and amplification strategies.

Conclusions:

Impedance measurements offer a powerful label-free approach for biosensing.
The described methods enable sensitive detection of biomarkers and analytes.
This technique holds significant potential for clinical diagnostics and analytical applications.