Related Experiment Video
Updated: Jul 10, 2026
07:04
Genotyping of Sea Anemone during Early Development
Published on: May 13, 2019
[AFLP analysis for genetic diversity of Ganoderma]
Lin-yong Zheng1, Ding-hong Jia, Xia Luo
1Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of life Sciences, Sichuan University, Chengdu 610064, China.
Summary
Genetic diversity in Ganoderma strains was analyzed using Amplified Fragment Length Polymorphism (AFLP). Results revealed significant molecular diversity, enabling clear differentiation between Ganoderma lucidum and Ganoderma sinense groups.
Area of Science:
- Mycology
- Molecular Biology
- Genetics
Background:
- Ganoderma species are vital medicinal mushrooms.
- Accurate identification and understanding of genetic diversity are crucial for cultivation and breeding programs.
Purpose of the Study:
- To assess the genetic diversity within Ganoderma cultivars.
- To enable accurate identification and classification of Ganoderma strains.
- To provide foundational data for breeding and cultivation.
Main Methods:
- Utilized Amplified Fragment Length Polymorphism (AFLP) analysis.
- Studied 24 Ganoderma lucidum and Ganoderma sinense samples.
- Employed NTSYSpc 2.1 software to construct a dendrogram.
Main Results:
- Generated 177 polymorphic bands across 14 primer combinations.
- Achieved unambiguous identification of all tested Ganoderma materials via AFLP.
- Demonstrated substantial genetic variation at the molecular level.
Conclusions:
- Significant genetic diversity exists among Ganoderma germplasm resources.
- All analyzed strains were successfully clustered into G. lucidum and G. sinense groups at a similarity coefficient of 0.676.
- AFLP is an effective tool for differentiating Ganoderma species and assessing genetic diversity.
