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Related Experiment Videos

Trehalose-transporting membrane vesicles from yeasts.

P S De Araujo1, A C Panek, J H Crowe

  • 1Department of Biochemistry, University of Sao Paulo, Brazil.

Biochemistry International
|July 1, 1991
PubMed
Summary
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Yeast membrane vesicles efficiently transport trehalose, mirroring intact cell kinetics. This transport relies on a pH gradient, crucial for retaining trehalose within vesicles.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Microbiology

Background:

  • Trehalose is a vital disaccharide in yeast, offering stress protection.
  • Understanding trehalose transport mechanisms is key to yeast physiology and biotechnology.

Purpose of the Study:

  • To isolate and characterize a yeast membrane vesicle fraction capable of trehalose transport.
  • To investigate the kinetic properties and regulatory factors of this transport system.

Main Methods:

  • Isolation of membrane vesicle fractions from yeast.
  • Measurement of trehalose uptake and release kinetics.
  • Manipulation of transmembrane pH gradients to assess transport dependence.

Main Results:

Related Experiment Videos

  • A functional membrane vesicle fraction capable of trehalose transport was successfully isolated.
  • The transport kinetics in vesicles closely resembled those in intact yeast cells.
  • Trehalose transport was dependent on a transmembrane pH gradient; dissipation led to leakage.
  • Vesicle transport activity diminished over time but could be partially restored by increasing internal pH.

    • Conclusions:

    • Yeast membrane vesicles provide a valuable model for studying trehalose transport.
    • A transmembrane pH gradient is essential for efficient trehalose accumulation in yeast vesicles.
    • The observed transport system exhibits characteristics consistent with active transport mechanisms.