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Broad spectrum of antibacterial activity of a new multipurpose disinfecting solution
Hua Zhu1, Aidong Ding, Mahesh Bandara
1Institute for Eye Research, School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.
Purpose:
Contact lens-related corneal infection and inflammation have been associated with microbial contamination of the contact lens or contact lens storage case. Antimicrobial performance of contact lens disinfection systems is an important factor in reducing contamination. The purpose of this study was to evaluate the antimicrobial efficacy of a new multipurpose disinfecting solution (MPDS), preserved with 0.001% polyquaternium-1 and 0.0005% myristamidopropyl dimethylamine, against a range of bacteria and fungi.
Methods:
The MPDS was challenged with a broad range of clinical and environmental isolates, including 10 gram-positive and eight gram-negative bacterial strains and three strains of fungi. The panel of reference microorganisms recommended by the International Organization for Standardization standards was also included. Samples were removed for analysis after 4 hours, 6 hours, or 24 hours of exposure to the MPDS. The number of survivors was determined by plate counts.
Results:
The new MPDS showed antimicrobial activity against the five reference microorganisms in excess of that recommended by International Organization for Standardization standards. The solution showed a broad spectrum of antibacterial activity, showing more than a 3 log reduction (mean, 4.2 +/- 1.4) in 8 of 10 gram-positive bacteria and more than a 4 log reduction (mean, 5.3 +/- 0.5) in all eight gram-negative bacteria at the 6-hour disinfection time. The efficacy of the solution was increased with longer exposure times (P<0.01). Some differences in activity between clinical and the reference bacterial strains were observed. The solution exceeded the 1 log unit reduction for all fungal species tested.
Conclusions:
The new MPDS produced a significant reduction in the growth of various clinical and environmental bacteria and fungi.
Insights
A new multipurpose disinfecting solution (MPDS) effectively reduces contamination on contact lenses. This antimicrobial solution demonstrated broad-spectrum efficacy against bacteria and fungi, enhancing contact lens safety.
Area of Science:
- Ophthalmology
- Microbiology
- Materials Science
Background:
- Contact lens wear is linked to microbial contamination, increasing risks of corneal infection and inflammation.
- Effective disinfection systems are crucial for preventing microbial contamination of contact lenses and storage cases.
Purpose of the Study:
- To evaluate the antimicrobial efficacy of a novel multipurpose disinfecting solution (MPDS).
- The MPDS contains 0.001% polyquaternium-1 and 0.0005% myristamidopropyl dimethylamine.
- To assess its effectiveness against a diverse range of bacteria and fungi.
Main Methods:
- The MPDS was tested against clinical and environmental isolates of gram-positive bacteria, gram-negative bacteria, and fungi.
- International Organization for Standardization (ISO) reference microorganisms were included in the challenge.
- Microbial survivors were quantified using plate counts after 4, 6, and 24 hours of exposure.
Main Results:
- The MPDS surpassed ISO standards for antimicrobial activity against reference microorganisms.
- It achieved >3-log reduction in 8/10 gram-positive bacteria and >4-log reduction in all 8 gram-negative bacteria within 6 hours.
- Antimicrobial efficacy increased with longer exposure times (P<0.01) and exceeded 1-log reduction for all tested fungi.
Conclusions:
- The new MPDS demonstrated significant antimicrobial activity against a wide array of clinical and environmental bacteria and fungi.
- This suggests its potential as an effective agent for contact lens disinfection.
- The solution offers enhanced safety for contact lens wearers by reducing microbial load.
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