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Updated: Jul 10, 2026

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
Published on: November 7, 2013
SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo
Christina Herzberg1, Lope Andrés Flórez Weidinger, Bastian Dörrbecker
1Department of General Microbiology, Georg-August-University Göttingen, Göttingen, Germany.
Abstract:
The detection and analysis of protein-protein interactions is one of the central tasks of proteomics in the postgenomic era. For this purpose, we present a procedure, the Strep-protein interaction experiment (SPINE) that combines the advantages of the Strep-tag protein purification system with those of reversible in vivo protein crosslinking by formaldehyde. Using two Bacillus subtilis regulator proteins, we demonstrate that this method is well suited to isolate protein complexes with high purity and virtually no background. Plasmids allowing the high-level expression of proteins carrying an N- or C-terminal Strep-tag in B. subtilis were constructed.
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