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Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans
Published on: April 28, 2023
Two novel biomarkers in developing wood frogs
Niti Gupta1, Amanda Hitchings, Lynn Weber
1University of Saskatchewan, Saskatoon, Saskatchewan, Canada. NJG792@mail.usask.ca
Integrated Environmental Assessment and Management
|November 13, 2007
Abstract:
Two novel biomarkers (whole-body thyroid hormone and triglyceride concentrations) were developed for use in wood frog tadpoles. The development of these biomarkers was based on previous work done in fish and lizards and incorporated triglyceride and thyroid hormone extraction into 1 homogenization step with 95% ethanol as the solvent. This method is useful for reducing sample-to-sample variability because the same sample can be used for each extraction and assay.
