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Updated: Jul 10, 2026

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy
Published on: July 1, 2021
Nonequilibrium fluctuations and mechanochemical couplings of a molecular motor
A W C Lau1, D Lacoste, K Mallick
1Department of Physics, Florida Atlantic University, 777 Glade Rd., Boca Raton, Florida 33431, USA.
Abstract:
We investigate theoretically the violations of Einstein and Onsager relations and the thermodynamic efficiency for a single processive motor operating far from equilibrium using an extension of the two-state model introduced by Kafri et al. [Biophys. J. 86, 3373 (2004)10.1529/biophysj.103.036152]. With the aid of the Fluctuation Theorem, we analyze the general features of these violations and this efficiency and link them to mechanochemical couplings of motors. In particular, an analysis of the experimental data of kinesin using our framework leads to interesting predictions that may serve as a guide for future experiments.
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