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Particles morphologically resembling mouse hepatitis virus in nude mouse uterus
Intervirology
|January 1, 1975
Summary
Particles resembling mouse hepatitis virus were found in nude mice. No oncornavirus-associated structures were detected, even after experimental inoculation with baboon type C viral materials.
Area of Science:
- Veterinary Pathology
- Virology
- Immunology
Background:
- Mouse hepatitis virus (MHV) is a common pathogen in laboratory mice.
- Nude mice are immunodeficient and susceptible to various infections.
- Oncornaviruses are a type of retrovirus associated with certain cancers.
Purpose of the Study:
- To investigate the presence of viral particles in the reproductive tract of nude mice.
- To determine if experimental inoculation with baboon type C viral material leads to observable ultrastructural changes.
Main Methods:
- Electron microscopy was used to examine uterine tissues from nude mice.
- Histopathological analysis of uterine gland lumens and endometrial cells.
- Comparison of tissues from control and experimentally inoculated mice.
Main Results:
- Particles morphologically similar to mouse hepatitis virus were identified in the uterine tissues of 8 nude mice.
- No ultrastructural evidence of oncornavirus-associated structures was found in any of the examined mice.
- Experimental inoculation with baboon type C viral-infected materials did not result in detectable oncornavirus structures.
Conclusions:
- The observed particles in nude mice warrant further investigation to confirm their identity as mouse hepatitis virus.
- The study did not find evidence of oncornavirus replication or associated structures in the reproductive tract of these mice following experimental inoculation.