Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eicosanoids mediate insect hemocyte migration.

Deepali Merchant1, Ronald L Ertl, Stephen I Rennard

  • 1Department of Biological Sciences, Northern Illinois University, DeKalb, IL, USA.

Journal of Insect Physiology
|November 13, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AT-HOME ASSESSMENT OF USABILITY AND DATA COLLECTION FROM A WEARABLE BIOELECTRONIC MONITORING DEVICE.

Proceedings of the ... Design of Medical Devices Conference. Design of Medical Devices Conference·2026
Same author

A Multidimensional Diagnostic Approach for Chronic Obstructive Pulmonary Disease.

JAMA·2025
Same author

Characteristics Associated with Lung Function Trajectories: An Analysis of the SPIROMICS Cohort.

Annals of the American Thoracic Society·2025
Same author

Phenotypes and Trajectories of Tobacco-exposed Persons with Preserved Spirometry: Insights from Lung Volumes.

Annals of the American Thoracic Society·2024
Same author

Polygenic and transcriptional risk scores identify chronic obstructive pulmonary disease subtypes in the COPDGene and ECLIPSE cohort studies.

EBioMedicine·2024
Same author

Differential interactions of ethacrynic acid and diethyl maleate with glutathione S-transferases and their glutathione co-factor in the house fly.

Pesticide biochemistry and physiology·2024
Same journal

A sex pheromone isomer modulates pheromone receptor sensitivity in Bombyx mori.

Journal of insect physiology·2026
Same journal

Peripheral coupling between Ca<sup>2+</sup> dynamics and neural output in tarsal gustatory sensilla of Bactrocera dorsalis.

Journal of insect physiology·2026
Same journal

Mean warming enhances insect resilience to high temperatures: species-specific evidence from two wheat aphids.

Journal of insect physiology·2026
Same journal

Biosynthetic pathway of juvenile hormone III skipped bisepoxide in the stink bug Plautia stali (Hemiptera: Pentatomidae).

Journal of insect physiology·2026
Same journal

Dietary lipids shape honey bee (Apis mellifera) feeding and resilience to pesticide stress.

Journal of insect physiology·2026
Same journal

A novel metabolic complementation pattern: The synergistic response between Sogatella furcifera and its symbionts under chitosan oligosaccharide stress sprayed on rice.

Journal of insect physiology·2026
See all related articles

Insect hemocytes detect N-formyl-Met-Leu-Phe (fMLP) and migrate toward it. Pharmaceutical inhibition of eicosanoid biosynthesis, involving cyclooxygenase (COX) and phospholipase A2 (PLA2), significantly reduces this essential insect immune cell migration.

Area of Science:

  • Insect immunology
  • Cellular and molecular biology
  • Biochemistry

Background:

  • Hemocyte migration is crucial for insect defense against infection and wounds.
  • The specific biochemical signals guiding insect hemocyte migration remain largely unknown.
  • N-formyl-Met-Leu-Phe (fMLP) is a key bacterial chemotactic peptide.

Purpose of the Study:

  • To investigate if insect hemocytes can detect and migrate towards fMLP.
  • To determine if pharmaceutical modulation of eicosanoid biosynthesis affects hemocyte migration.
  • To elucidate the role of eicosanoids in mediating insect hemocyte chemotaxis.

Main Methods:

  • Primary hemocyte cultures from Manduca sexta (tobacco hornworm) were used.
  • Boyden chambers assessed hemocyte migration towards buffer (control) and fMLP (chemoattractant).

Related Experiment Videos

  • Inhibitors of cyclooxygenase (COX), lipoxygenase, and phospholipase A2 (PLA2) were administered to hornworms prior to culture.

    • Main Results:

    • Hemocyte migration towards fMLP was significantly higher (64%) compared to buffer (42%).
    • Pre-treatment with COX, lipoxygenase, and PLA2 inhibitors reduced hemocyte migration by over 40%.
    • Dexamethasone's inhibitory effect was dose-dependent and reversible by arachidonic acid, but not palmitic acid.

    Conclusions:

    • Insect hemocytes possess the ability to detect and migrate in response to fMLP.
    • Eicosanoid biosynthesis pathways, regulated by COX and PLA2, are essential mediators of insect hemocyte migration.
    • These findings provide critical insights into the biochemical mechanisms of insect innate immunity.