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Related Concept Videos

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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Various dissolution methods are utilized to assess a drug’s dissolution rate, including the flow-through cell, paddle-over-disk, cylinder, and reciprocating disk methods.The flow-through cell apparatus (USP (United States Pharmacopeia) method 4) comprises a reservoir for the dissolution medium and a pump that propels the medium through the cell containing the test sample. This method is crucial for assessing modified-release dosage forms with minimally soluble active ingredients, maintaining...
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Updated: Jul 10, 2026

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
07:47

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

Published on: April 20, 2015

High throughput methods to characterize protein permeation and release.

Martinus A H Capelle1, Robert Gurny, Tudor Arvinte

  • 1Department of Pharmaceutics and Biopharmaceutics,University of Geneva, University of Lausanne, CH-1211 Geneva 4, Switzerland.

International Journal of Pharmaceutics
|November 13, 2007
PubMed
Summary

New spectroscopic methods in multi-well plates enable high-throughput study of protein permeation and drug release kinetics. These techniques efficiently characterize protein transport and drug delivery systems using minimal sample volumes.

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18:57

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Published on: October 17, 2013

Area of Science:

  • Biophysical Chemistry
  • Drug Delivery Systems
  • Spectroscopic Analysis

Background:

  • Protein permeation and release kinetics are crucial for drug delivery system characterization.
  • Traditional methods can be time-consuming and require large sample volumes.
  • Development of high-throughput screening methods is essential for efficient drug development.

Purpose of the Study:

  • To develop and validate spectroscopic methods for studying protein permeation and release kinetics in multi-well plates.
  • To characterize the permeation of bovine serum albumin (BSA) through membranes with varying pore characteristics.
  • To investigate the release kinetics of a model protein drug, hirudin, from an agar hydrogel formulation.

Main Methods:

  • Utilized 96-well plates with a membrane separating two compartments for permeation studies.
  • Measured fluorescence intensity changes to quantify bovine serum albumin (BSA) permeation.
  • Formulated hirudin in an agar hydrogel within multi-well plates and monitored release via intrinsic tyrosine fluorescence over 12 hours.

Main Results:

  • Protein permeation was found to be dependent on membrane pore size and density.
  • Spectroscopic analysis successfully monitored hirudin release from the agar hydrogel at different locations.
  • The developed methods demonstrated sensitivity to changes in protein release kinetics.

Conclusions:

  • High-throughput spectroscopic methods are effective for characterizing protein permeation and drug release from delivery systems.
  • These methods allow for the use of small sample volumes, increasing efficiency.
  • The study validates the application of multi-well plate-based assays for rapid screening of protein drug delivery systems.