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Updated: Jul 10, 2026

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
Published on: April 22, 2022
Oligothiophene-5-labeled deoxyuridines for the detection of single nucleotide polymorphisms
Massimo L Capobianco1, Antonella Cazzato, Silvia Alesi
1Istituto per la Sintesi Organica e la Fotoreattività del Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129 Bologna, Italy. capobianco@isof.cnr.it
Abstract:
We report the synthesis of four oligothiophene-5-labeled deoxyuridines. These modified fluorescent nucleosides have been incorporated into oligodeoxynucleotides designed to be used as probes to discriminate, through changes in fluorescence emission, between hybridization with a perfectly complementary strand and that with a single nucleotide mismatch facing the modified uridines. Upon hybridization, remarkable differences (up to 47%) of the emitted light, depending on the uridine facing base, were observed.
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