Related Experiment Video
Updated: Jul 10, 2026
12:02
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
Knockdown of human MCM10 activates G2 checkpoint pathway
Jong Hoon Park1, Sung Woong Bang, Sung Ho Kim
1Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Biochemical and Biophysical Research Communications
|November 14, 2007
Summary
MCM10 protein is crucial for DNA replication and cell cycle progression. Its depletion causes replication delays, activating cell cycle checkpoints and leading to DNA damage and cell death, highlighting its role in genome integrity.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- MCM10 is a known component of chromosome replication elongation in model organisms.
- Previous work demonstrated that human MCM10 knockdown causes delayed and incomplete DNA replication.
Purpose of the Study:
- To investigate the cell cycle consequences of delayed and incomplete DNA replication due to MCM10 knockdown.
- To elucidate the role of MCM10 in maintaining genome integrity and cell cycle progression.
Main Methods:
- Human MCM10 protein knockdown was performed.
- Cell cycle progression and checkpoint activation were analyzed.
- DNA damage and cell death were assessed.
Main Results:
- MCM10 knockdown activated a Chk1/Cdc25-mediated checkpoint pathway, inhibiting Cdk1.
- Cdk1 inhibition led to G2 phase arrest in MCM10-depleted cells.
- Prolonged MCM10 depletion resulted in significant DNA damage and subsequent cell death.
Conclusions:
- MCM10 is essential for timely and complete chromosome replication.
- MCM10 plays a critical role in preventing DNA damage and maintaining genome stability.
- MCM10 is vital for proper cell cycle progression, particularly the G2 to M transition.
Related Concept Videos
02:36DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
01:23Negative Regulator Molecules
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...