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Updated: Jul 10, 2026
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Generation and Functional Verification of Hypoxia-Sensitive Chimeric Antigen Receptor-T Cells
Published on: June 14, 2024
HIF gene expression in cancer therapy
Denise A Chan1, Adam J Krieg, Sandra Turcotte
1Department of Radiation Oncology, Stanford University School of Medicine, Stanford, California, USA.
Methods in Enzymology
|November 14, 2007
Summary
Tumor hypoxia, a common condition in solid tumors, involves the hypoxia-inducible factor (HIF) pathway. This review explores HIF transcriptional activity and its potential for cancer therapy.
Area of Science:
- Oncology
- Molecular Biology
- Cellular Physiology
Background:
- Tumor hypoxia is prevalent in solid tumors due to abnormal vasculature.
- Tumor cells adapt to low-oxygen environments via specific mechanisms.
- The hypoxia-inducible factor (HIF) family, comprising alpha and beta subunits, regulates cellular responses to hypoxia.
Purpose of the Study:
- To review the study of HIF transcriptional activity.
- To explore the exploitation of HIF in cancer therapy.
Main Methods:
- Focus on the transcriptional regulation by HIF.
- Investigate mechanisms controlling HIF levels and activity.
Main Results:
- HIF binds to genomic sequences, upregulating genes essential for hypoxic adaptation.
- Multiple regulatory mechanisms control HIF levels and transcriptional activity.
Conclusions:
- HIF plays a critical role in maintaining oxygen homeostasis.
- Understanding HIF transcriptional activity is key for developing novel cancer therapies.
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