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Related Experiment Video

Updated: Jul 10, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

DNA methylation analysis as a tool for cell typing.

Udo Baron1, Ivana Türbachova, Alexander Hellwag

  • 1Epiontis GmbH, Berlin, Germany.

Epigenetics
|November 14, 2007
PubMed
Summary
This summary is machine-generated.

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DNA methylation patterns can serve as cell type-specific markers for quality control in cell therapies. This methylation-based analytics approach provides unambiguous profiles for cell identification and quantification of cell mixtures.

Area of Science:

  • Biotechnology
  • Genomics
  • Cell Biology

Background:

  • Cell therapeutic approaches require robust quality control for safety and regulatory approval.
  • Ex vivo cell manipulation can lead to heterogeneity and deviations from natural growth processes.
  • Accurate cell identity verification and subpopulation quantification are critical.

Purpose of the Study:

  • To investigate DNA methylation patterns as distinctive cell type markers for cultured human primary cells.
  • To develop a method for unambiguous cell type profiling and quantification.
  • To establish methylation-based analytics for quality control in cell-based therapies.

Main Methods:

  • Identification of cell type-specific differentially methylated gene regions (CDMs).
  • Application of methylation-sensitive single nucleotide primer extension.

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Methodology for Accurate Detection of Mitochondrial DNA Methylation
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Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
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Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

Published on: February 19, 2020

Related Experiment Videos

Last Updated: Jul 10, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
08:02

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

Published on: February 19, 2020

  • Generation of binary cell type descriptors ('barcodes') for quantification.

    • Main Results:

    • Distinctive DNA methylation patterns were identified for different human primary cell types.
    • CDMs generated unambiguous profiles, serving as reliable cell type markers.
    • The developed method allowed for the quantification of cell mixtures.

    Conclusions:

    • DNA methylation patterns are effective markers for cell type characterization.
    • Methylation-based analytics offer a promising tool for quality control of ex vivo manipulated cells.
    • This approach can enhance the safety and consistency of cell therapies.