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Updated: Jul 10, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Using LUMA: a Luminometric-based assay for global DNA-methylation.

Mohsen Karimi¹, Sofia Johansson, Tomas J Ekström

¹Department of Clinical Neuroscience, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.

|November 14, 2007

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A new method, LUminometric Methylation Assay (LUMA), quantifies global genomic DNA methylation using restriction digestion and Pyrosequencing. This rapid, reproducible technique is valuable for epigenetic research and clinical applications.

Area of Science:

Epigenetics
Molecular Biology
Genomics

Background:

Genomic DNA methylation is crucial for cellular processes, development, differentiation, and diseases like cancer.
Understanding global DNA methylation patterns is vital for biological and medical research.

Purpose of the Study:

To introduce a novel, quantitative method for analyzing global genomic DNA methylation.
To provide a detailed description and example application of the LUminometric Methylation Assay (LUMA).

Main Methods:

Developed the LUminometric Methylation Assay (LUMA) using luminometric technology for methylation-sensitive restriction digestion.
Utilized a polymerase extension assay on the Pyrosequencing platform for quantitation.
Incorporated an internal control for DNA input, requiring no DNA modification.

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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

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Published on: June 16, 2017

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Main Results:

LUMA is a quantitative and highly reproducible method for analyzing global DNA methylation.
The assay has a total running time of only six hours.
Demonstrated suitability for clinical material and dynamic methylation/demethylation event analysis.

Conclusions:

LUMA offers a rapid, reliable, and efficient approach to assess global DNA methylation.
The method is applicable to various research settings, including clinical studies and epigenetic research.
LUMA facilitates the study of dynamic changes in DNA methylation crucial for understanding health and disease.