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Related Experiment Video

Updated: Jul 10, 2026

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

Splenocytes can replace chimeric cells and maintain allograft tolerance.

Yuuki Hayashi¹, Shintaro Yamazaki, Akira Kanamoto

¹Department of Digestive Surgery, Nihon University School of Medicine, Tokyo, Japan.

Transplantation

|November 14, 2007

Summary

This summary is machine-generated.

Donor-specific tolerance (DST) can be achieved by establishing chimerism. Splenocyte infusion in chimeric mice maintained chimerism and prolonged skin graft survival, demonstrating a potential path to engraftment without immunosuppression.

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Area of Science:

Immunology
Transplantation Biology
Chimerism Research

Background:

Donor-specific tolerance (DST) is a novel strategy for facilitating engraftment without immunosuppression.
Establishing a chimeric state, where donor-derived cells coexist within the recipient, is a key approach to inducing DST.
The role of splenocytes in maintaining chimerism and prolonging graft survival requires further investigation.

Purpose of the Study:

To investigate the potential of splenocytes in maintaining chimerism.
To assess the impact of splenocyte administration on prolonging skin graft survival in a chimeric model.

Main Methods:

A mixed bone marrow (BM) chimeric model was established in lethally irradiated C3H mice.
Mice received BM transplants from C3H and B6D2F1 strains, followed by C57BL/6 skin grafts.
Splenocytes from C3H, B6D2F1, or B6C3F1 mice were administered to chimeric recipients to assess chimerism and graft survival.

Main Results:

Splenocyte infusion in group B (B6D2F1) and group C (B6C3F1) led to the establishment and maintenance of chimerism, with B6D2F1-derived cells reaching 99.6% and B6C3F1-derived cells reaching 77.8% respectively.
In contrast, BM-derived chimeric cells were eliminated in group A (C3H).
Skin grafts exhibited prolonged survival, lasting at least 200 days in groups B and C.

Conclusions:

Chimerism established with cells sharing a partial Major Histocompatibility Complex (MHC) match with the graft can maintain specific immunotolerance.
This approach effectively prolongs graft survival, offering a promising strategy for transplantation.