Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Compact microfluidic structures for generating spatial and temporal gradients.

Dragos Amarie1, James A Glazier, Stephen C Jacobson

  • 1Department of Chemistry, Indiana University, Bloomington, Indiana 47405-7102, USA.

Analytical Chemistry
|November 15, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient septum formation is essential for chromosome segregation in <i>Bacillus subtilis</i> when SMC function is impaired.

bioRxiv : the preprint server for biology·2026
Same author

Modeling epithelial deformation and cell rearrangement in response to external forces during Zebrafish epiboly.

NPJ systems biology and applications·2026
Same author

Who's afraid of synthetic data? Hybrid approaches to deliver medical digital twins.

Informatics in medicine unlocked·2026
Same author

From FAIR to CURE: guidelines for computational models of biological systems.

NPJ systems biology and applications·2026
Same author

Constitutive, endogenous, fluorescent membrane reporters for dynamic cell cycle analysis in <i>Bacillus subtilis</i>.

bioRxiv : the preprint server for biology·2026
Same author

V-Cornea: A computational model of corneal epithelium homeostasis, injury, and recovery.

PLoS computational biology·2025
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026
Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
See all related articles

This study introduces a compact microfluidic device for generating precise spatial and temporal gradients. The novel design enables rapid switching between gradients, reducing reagent use and allowing complex experimental sequences.

Area of Science:

  • Microfluidics
  • Biotechnology
  • Chemical Engineering

Background:

  • Generating precise chemical gradients is crucial for studying cellular responses.
  • Existing microfluidic gradient generators can be bulky and slow to switch compositions.

Purpose of the Study:

  • To develop an improved microfluidic design for generating spatial and temporal gradients.
  • To create a compact and efficient gradient generator with rapid switching capabilities.

Main Methods:

  • Utilized bifurcated and trifurcated channels for flow splitting and mixing tees for recombination.
  • Designed a compact gradient-forming structure with 2(N) + 1 discrete steps for N gradient levels.
  • Fabricated and tested devices with varying channel dimensions and gradient levels.

Related Experiment Videos

Main Results:

  • Achieved a compact gradient generator (1.6 mm x 0.5 mm) enabling 2(N) + 1 discrete gradient steps.
  • Demonstrated rapid switching between gradients in 2.6 seconds, reducing reagent consumption.
  • Successfully generated high-quality linear gradients using both pressure-driven and electrokinetic flow.

Conclusions:

  • The improved microfluidic design offers a significant advancement in generating spatial and temporal gradients.
  • The compact size and fast switching capabilities facilitate complex temporal gradient experiments and reduce reagent waste.
  • This technology supports diverse applications requiring precise control over chemical environments.