Related Experiment Video

Updated: Jul 10, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Involvement of notch signaling in wound healing

Srinivasulu Chigurupati1, Thiruma V Arumugam, Tae Gen Son

  • 1Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, Maryland, United States of America.

Plos One
|November 15, 2007
PubMed

Insights

Notch signaling inhibition delays wound healing, while its activation enhances it. Targeting this pathway could offer new strategies for wound treatment and tissue repair.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Tissue Repair

Background:

  • The Notch signaling pathway regulates cell fate decisions during tissue development.
  • Its role in wound healing and tissue repair remains to be fully elucidated.

Purpose of the Study:

  • To investigate the role of Notch signaling in the process of wound healing.
  • To determine if modulating Notch signaling can impact dermal wound repair and angiogenesis.

Main Methods:

  • Utilized in vivo models (Notch antisense transgenic mice, gamma-secretase inhibitors, Jagged peptide treatment) and cell culture models.
  • Assessed full-thickness dermal wound healing and scratch wound assays in cultured cells (endothelial cells, keratinocytes, fibroblasts).

Main Results:

  • Inhibition of Notch signaling (via antisense or gamma-secretase inhibitors) significantly delayed wound healing.
  • Activation of Notch signaling (via Jagged peptide) significantly enhanced wound healing.
  • Modulation of Notch signaling affected the behavior of vascular endothelial cells, keratinocytes, and fibroblasts in wound healing models.

Conclusions:

  • Notch signaling plays a crucial role in wound healing and tissue repair.
  • Targeting the Notch pathway presents a potential novel therapeutic strategy for wound treatment.
  • Modulation of Notch signaling may also be beneficial for controlling angiogenesis in pathological conditions.

More Related Videos

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis06:36

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis

Published on: August 21, 2020

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Phases of Wound Repair01:28

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...