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Ligand-modified aminobisphosphonate for linking proteins to hydroxyapatite and bone surface
Robin S Ehrick1, Marcello Capaccio, David A Puleo
1Department of Chemistry, University of Kentucky, Lexington, Kentucky 40506-0055, USA.
Bioconjugate Chemistry
|November 16, 2007
Summary
Modified aminobisphosphonates, like biotinylated versions, effectively bind to bone's mineral component, hydroxyapatite. This suggests potential for linking proteins and peptides to bone surfaces for osteoporosis treatment.
Area of Science:
- Biomaterials Science
- Orthopedics
- Drug Delivery
Background:
- Osteoporosis is characterized by increased bone resorption, a process targeted by bisphosphonates.
- Aminobisphosphonates offer a versatile conjugation point for attaching molecules like proteins and peptides to bone.
- Developing effective bone-targeting agents is crucial for osteoporosis management.
Purpose of the Study:
- To investigate the binding of biotinylated aminobisphosphonate to hydroxyapatite, the primary bone mineral.
- To evaluate the potential of modified aminobisphosphonates as linkers for protein attachment to bone surfaces.
- To assess the interaction of biotinylated aminobisphosphonate with cortical bone samples.
Main Methods:
- Synthesis of an aminobisphosphonate conjugated with biotin.
- Quantification of binding to hydroxyapatite using a fluorescently labeled anti-biotin antibody.
- In vitro study of biotinylated aminobisphosphonate interaction with cortical bone from a cow femur.
Main Results:
- Biotinylated aminobisphosphonate demonstrated significant binding to hydroxyapatite.
- The biotin functionality remained accessible for antibody recognition after binding.
- Modified aminobisphosphonates showed high-level binding to both hydroxyapatite and cortical bone samples.
Conclusions:
- Modified aminobisphosphonates can effectively bind to bone mineral and bone tissue.
- The biotin tag remains available for further molecular conjugation, enabling targeted delivery.
- This approach holds promise for developing novel bone-targeting therapies for osteoporosis and other bone diseases.
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