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Super-resolution Fluorescence Microscopy

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Related Experiment Video

Updated: Jul 10, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Fluorescence diffuse optical tomographic (fDOT) system for small animal studies.

A Koenig¹, J Boutet, L Hervé

¹CEA-LETI Minatec, DTBS/STD, 17 rue des Martyrs, 38054 Grenoble cedex France. anne.koenig@cea.fr

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

|November 16, 2007

Summary

This summary is machine-generated.

A new fluorescence diffuse optical tomography (fDOT) system reconstructs fluorescence yield in challenging tissues without immersion. This enables effective biological studies in small animals, including tumor development tracking.

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Area of Science:

Biomedical Optics
Medical Imaging
Optical Tomography

Background:

Fluorescence diffuse optical tomography (fDOT) is crucial for in vivo imaging.
Imaging in heterogeneous and highly attenuating tissues presents significant challenges.
Current methods often require sample immersion in index-matching fluids.

Purpose of the Study:

To introduce a novel fDOT system and reconstruction algorithm.
To enable fluorescence yield reconstruction in complex biological tissues.
To facilitate small animal imaging without immersion media.

Main Methods:

Development of a new fDOT system.
Implementation of an advanced reconstruction algorithm for heterogeneous media.
Phantom studies for system characterization and validation.
In vivo mice study focusing on lung tumor development.

Main Results:

The fDOT system successfully reconstructs fluorescence yield in heterogeneous and attenuating regions.
The method allows for mouse imaging without optical index matching liquid (Intralipid and ink).
Phantom experiments validated the system's performance in complex media.
A mice study demonstrated effective follow-up of lung tumor development.

Conclusions:

The developed fDOT system and reconstruction method are effective for imaging challenging biological tissues.
The system eliminates the need for immersion media, simplifying small animal studies.
This technology is validated for biological applications, particularly in tracking tumor progression in vivo.