Chromium hexacyanoferrate modified biosensor based on PQQ-dependent glucose dehydrogenase

Area of Science:

• Electrochemistry
• Biosensor Technology
• Biotechnology

Background:

• Glucose detection is critical for managing diabetes and other metabolic disorders.
• Enzyme-based biosensors offer high specificity for glucose but often suffer from limited stability.
• Developing robust and long-lasting glucose biosensors remains a significant challenge.

Purpose of the Study:

• To develop a novel electrochemical biosensor for sensitive glucose detection.
• To enhance electron transfer efficiency and sensor stability using mediator modification.
• To evaluate the performance and operational lifespan of the developed glucose biosensor.

Main Methods:

• Fabrication of a platinum electrode modified with Pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase.
• Incorporation of chromium hexacyanoferrate as an electron transfer mediator.
• Optimization of detection parameters including pH and applied potential.
• Assessment of sensor stability and enzyme activity over an extended period.

Main Results:

• The PQQ-dependent glucose dehydrogenase biosensor exhibited optimal performance at pH 7 and +0.4 V (vs. Ag/AgCl).
• Chromium hexacyanoferrate effectively mediated electron transfer between PQQ and the platinum electrode.
• The developed biosensor demonstrated remarkable stability, maintaining enzyme activity for 47 days without significant degradation.

Conclusions:

• The developed PQQ-based glucose biosensor offers a promising platform for accurate and stable glucose detection.
• The use of chromium hexacyanoferrate as a mediator significantly enhances biosensor performance and longevity.
• This stable and efficient biosensor holds potential for various diagnostic and monitoring applications.