Related Experiment Video
Updated: Jul 10, 2026
10:28
Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
Published on: June 30, 2016
Quantitative analysis method to evaluate optical clearing effect of skin using a hyperosmotic chemical agent
Jinhee Yoon1, Taeyoon Son, Byungjo Jung
1Department of Biomedical Engineering, Yonsei University, South Korea. no-fate@yonsei.ac.kr
Summary
This study demonstrates glycerol's effectiveness in enhancing light penetration in scattering tissues like skin. A novel quantitative method is introduced to assess this optical clearing effect spatially.
Area of Science:
- Biomedical Optics
- Tissue Optics
- Photomedicine
Background:
- Light penetration depth in highly scattering tissues is crucial for optical imaging and therapy.
- Hyperosmotic agents like glycerol, polyethylene glycol (PEG), and glucose are known to enhance light penetration.
- Previous studies have quantitatively evaluated skin optical clearing using OCT, spectroscopy, and integrating sphere methods.
Purpose of the Study:
- To demonstrate the optical clearing effect of glycerol on skin.
- To introduce and validate a new quantitative analysis method for evaluating the spatial optical clearing effect of skin treated with glycerol.
Main Methods:
- Application of glycerol to skin tissue.
- Quantitative analysis of spatial optical clearing effect using a novel method.
Main Results:
- Glycerol effectively enhances light penetration depth in scattering skin tissues.
- The proposed quantitative analysis method successfully evaluates the spatial optical clearing effect.
Conclusions:
- Glycerol is a viable agent for improving light penetration in skin.
- The developed quantitative method provides a new tool for assessing skin optical clearing spatially.
