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Updated: Jul 10, 2026

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Published on: November 29, 2016
A comparative study of adaptive registration methods for two dimensional gel electrophoresis images
Huamei Xin1, Yuemin Zhu, Longfei Wu
1CREATIS-LRMN, CNRS, UMR 5220, Villeurbanne, F-69621, France. huamei.xin@creatis.insa-lyon.fr
Abstract:
Two-dimensional gel electrophoresis (2DGE) images play a major role in techniques for protein separation. The registration of 2DGE images is considered as one of key elements in protein identification. This paper proposes a single adaptive registration scheme for 2DGE images. Within the scheme, three registration methods based on the combined use of global linear affine and local nonlinear thin-plate spline (TPS), Demons, B-spline algorithms are explored and compared. The results show that the method using multi-resolution B-spline is preferred.
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