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Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
[Effect of Erigeron Breviscapine on premaxillary suture distraction osteogenesis in rabbit]
1Department of Orthodontics, Stomatology Hospital, Xi'an Jiaotong University. Xi'an 710004, Shanxi Province, China. zoumin@mail.xjtu.edu.cn
Purpose:
The aim of this study is to determine the effects of Erigeron Breviscapine (EB) in suture distraction osteogenesis (DO) in the maxillary protraction models of rabbit.
Methods:
In distraction study, the rabbits were randomly divided into 2 groups based on protracted or unprotracted (n=16, 8 in each). The cephalograms were taken in the 0 and the 28th day. In EB study, all samples were protracted and were randomly divided into 2 groups based on injection of EB or NaCl. The width of serial tetracycline fluorochrome was labeled and measured during 28 days (n=16, 8 in each). In the 7th and the 14th day, the tissues of the premaxillary bone suture were harvested and processed for immunohistochemistry of TGF-beta (n=12, 6 in each). Group t test was performed by using SPSS10.0 software package.
Results:
It was found that the cephalometric parameters of the sagittal maxilla were significantly increased in the protraction group after 28 days (P<0.05). The width of tetracycline fluorochrome was significantly increased in the EB group after 28 days (P<0.05). In the same time, there was stronger expression in EB group than in NaCl group (P<0.05). There was stronger TGF-beta expression in the 7th day EB group than in the 14th day NaCl group (P<0.05). There were abundant fibroblast and blood vessels in the active DO. There were not typical active osteoblasts in the border of the bone sutures.
Conclusions:
Maxillary protraction models of rabbit is successfully established. EB can accelerate DO. One reason of EB accelerating DO may be angiogenesis and improvement of microcirculation.Direct osteogenesis of EB is also possible. Supported by Science and Technology Development Project of Shanxi Province [Grant No.2002K11-G7(3)].
