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Microfluidic cell electroporation using a mechanical valve.

Jun Wang1, M Jane Stine, Chang Lu

  • 1Department of Agricultural and Biological Engineering, Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907, USA.

Analytical Chemistry
|November 17, 2007
PubMed
Summary
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This study presents a novel microfluidic electroporation method using an elastomeric valve to generate electrical pulses for cell membrane permeabilization. This cost-effective technique simplifies cell electropermeabilization for microfluidic applications.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Electroporation is crucial for cell membrane manipulation.
  • Existing microscale electroporation devices often require complex pulse generators and electrodes.

Purpose of the Study:

  • To demonstrate a simplified microfluidic electroporation technique.
  • To reduce the cost and complexity of cell membrane permeabilization in microfluidic systems.

Main Methods:

  • Fabrication of a microfluidic chip using poly(dimethylsiloxane) (PDMS).
  • Integration of an elastomeric valve to control electrical circuit interruption.
  • Utilizing a common DC power supply for electroporation.

Main Results:

Related Experiment Videos

  • Successful electropermeabilization of Chinese hamster ovary (CHO) cells (suspended and adherent).
  • Demonstrated cell membrane permeabilization using SYTOX green DNA dye.
  • Eliminated the need for specialized pulse generators and microfabricated electrodes.

    • Conclusions:

    • The elastomeric valve-based microfluidic electroporation is a cost-effective and simplified alternative.
    • This technique is suitable for integration into larger microfluidic systems.
    • Offers potential for scalable cell poration in biotechnology.