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Determination of calcium ions tightly bound to proteins
1Department of Polymer Science, Faculty of Science, Hokkaido University, Japan.
Journal of Chromatography
|October 25, 1991
Abstract:
A rapid and sensitive procedure is described for the determination of calcium ions tightly bound to proteins using high-performance gel filtration chromatography, followed by the destabilization of the protein conformation and fluorimetric analysis with Quin-2. With this method, contaminating, unbound calcium can be eliminated simultaneously and one can determine the content of a calcium ion in a protein utilizing less than 200 pmol of the protein.