Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Designer bugs: structural engineering to build a better mouse model.

Shyam S Bhaskaran1, C Erec Stebbins

  • 1Laboratory of Structural Microbiology, Rockefeller University, New York, NY 10021, USA.

Cell Host & Microbe
|November 17, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The fentanyl-specific antibody FenAb024 can shield against carfentanil effects.

Toxicology letters·2024
Same author

A structural classification of the variant surface glycoproteins of the African trypanosome.

PLoS neglected tropical diseases·2023
Same author

Immunodominant surface epitopes power immune evasion in the African trypanosome.

Cell reports·2023
Same author

Structural similarities between the metacyclic and bloodstream form variant surface glycoproteins of the African trypanosome.

PLoS neglected tropical diseases·2023
Same author

A trypanosome-derived immunotherapeutics platform elicits potent high-affinity antibodies, negating the effects of the synthetic opioid fentanyl.

Cell reports·2023
Same author

Nanobody-mediated macromolecular crowding induces membrane fission and remodeling in the African trypanosome.

Cell reports·2021
Same journal

Gut commensal Bacteroides-derived pantothenic acid alleviates metabolic syndrome.

Cell host & microbe·2026
Same journal

Predicting antimicrobial resistance for precision medicine.

Cell host & microbe·2026
Same journal

Meta-analysis reveals microbiome signatures for colorectal cancer that are universal across age groups and sequencing methods.

Cell host & microbe·2026
Same journal

Single-cell detection and quantification of the microbiota by MicFLY.

Cell host & microbe·2026
Same journal

Suppression of host salicylic acid defense by a phloem-colonizing pathogen effector in citrus Huanglongbing.

Cell host & microbe·2026
Same journal

CBASS limits bacteriophage production while maintaining cell viability in Pseudomonas aeruginosa.

Cell host & microbe·2026
See all related articles

Researchers modified Listeria monocytogenes to better infect mice. This bacterial pathogen now invades intestinal cells, creating a more accurate animal model for studying bacterial diseases in humans.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Animal Models

Background:

  • Bacterial disease research is limited by animal models that don't accurately reflect human infection routes.
  • Differences in tissue tropism and pathogenesis course hinder the study of many bacterial diseases.

Purpose of the Study:

  • To develop a "murinized" Listeria monocytogenes strain that mimics human infection pathways in mice.
  • To overcome limitations of current animal models for bacterial pathogenesis research.

Main Methods:

  • Rational mutation of the bacterial surface invasion protein (InlA) in Listeria monocytogenes.
  • Enhancing the binding affinity of InlA to its host receptor, E-cadherin.
  • Testing the modified strain's ability to invade mouse intestinal epithelial cells.

Related Experiment Videos

Main Results:

  • A "murinized" Listeria monocytogenes strain was successfully created.
  • The modified strain demonstrated invasion of intestinal epithelial cells in mice.
  • This establishes a more accurate model for human Listeria monocytogenes infection.

Conclusions:

  • The engineered Listeria monocytogenes strain provides a valuable tool for studying bacterial pathogenesis.
  • This advancement improves the translational relevance of mouse models for human infectious diseases.
  • Further research can utilize this model to explore therapeutic strategies against bacterial infections.