Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative study of WO<sub>3</sub> and WS<sub>2</sub> nanoparticles in regulating antibiotic resistance gene transfer: Implications for differential roles of metal oxides and sulfides.

Journal of hazardous materials·2026
Same author

Interpretable Deep Learning for Single-Molecule Nanopore Fingerprinting Using Physics-Guided Preprocessing.

ACS sensors·2026
Same author

AI to Identify Strain-Sensitive Regions of the Optic Nerve Head Linked to Functional Loss in Glaucoma.

Investigative ophthalmology & visual science·2026
Same author

Modular generation of multispecific antibodies using protein trans-splicing.

mAbs·2025
Same author

Improving dual targeting selectivity in T-cell engagers via synapse-gated and affinity-tuned trispecific antibody design.

mAbs·2025
Same author

Training of physical neural networks.

Nature·2025

Related Experiment Video

Updated: Jul 10, 2026

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Real-time spectral imaging in three spatial dimensions.

Wenhai Liu, Demetri Psaltis, George Barbastathis

    Optics Letters
    |November 17, 2007
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a novel volume-holographic microscope. This instrument captures 3D spatial and spectral data of microscopic objects simultaneously, advancing optical imaging capabilities.

    More Related Videos

    Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and &lambda;) Hyperspectral FRET Imaging and Analysis
    08:22

    Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis

    Published on: October 27, 2020

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Related Experiment Videos

    Last Updated: Jul 10, 2026

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
    11:34

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and &lambda;) Hyperspectral FRET Imaging and Analysis
    08:22

    Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis

    Published on: October 27, 2020

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Area of Science:

    • Optical imaging
    • Microscopy
    • Holography

    Background:

    • Current microscopy techniques often struggle to capture comprehensive data from semitranslucent objects.
    • Simultaneous acquisition of spatial and spectral information is challenging in microscopic imaging.

    Purpose of the Study:

    • To introduce and characterize the first volume-holographic optical imaging instrument.
    • To demonstrate its capability for simultaneous 3D spatial and spectral data acquisition.

    Main Methods:

    • Development of a four-dimensional volume-holographic microscope.
    • Theoretical characterization of the instrument's performance.
    • Experimental validation using fluorescent microspheres as test objects.

    Main Results:

    • The instrument successfully returned three-dimensional spatial information.
    • Spectral information of microscopic objects was obtained in a single measurement.
    • Demonstrated the feasibility of volume-holographic microscopy for detailed object analysis.

    Conclusions:

    • The developed instrument represents a significant advancement in optical imaging.
    • This technology enables comprehensive analysis of semitranslucent microscopic objects.
    • Potential applications in various scientific fields requiring high-resolution, multi-dimensional imaging.