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Development of a Negative Selectable Marker for Entamoeba histolytica
Published on: December 12, 2010
A modification of Diamond's medium for the axenic culture of Entamoeba histolytica
C J Marinkelle1, F Guhl, A Aguirre
1Universidad de los Andes, Laboratorio de Microbiología y Parasitología, Bogotá, Colombia.
Transactions of the Royal Society of Tropical Medicine and Hygiene
|November 1, 1991
Abstract:
The use of casein hydrolysate in Diamond's axenic culture medium TPS-1 in replacement of trypticase allowed good growth of the trophozoites of Entamoeba histolytica. This modified medium also supported growth of trophozoites preserved for 16 months in liquid nitrogen. Considerable labour and cost of serum can be saved by using 5% instead of 10% bovine serum in combination with this modified medium.

