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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

[Immunochemical determination of human thiol protein disulfide oxidoreductase in cell and tissue homogenates by

H Kröning1, H W Mansfeld, C Held

  • 1Forschungsabteilung Experimentelle Immunologie, Medizinischen Akademie Magdeburg.

Allergie Und Immunologie
|January 1, 1991
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.