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Variants of human seminal acrosin inhibitor (HUSI-II) which inhibit human leukocyte elastase
Abstract:
A cDNA coding for human seminal inhibitor II was fused to the ompA-gene leader peptide for the expression in E. coli. The secreted protein is biologically active and correctly processed. Inhibitors of human leukocyte elastase were constructed by site-directed mutagenesis.