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Updated: Jul 10, 2026

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

[Recent progress in mycobacteriology].

Masaji Okada¹, Kazuo Kobayashi

¹Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, Japan.

Kekkaku : [Tuberculosis]

|November 21, 2007

Summary

New strategies for combating tuberculosis (TB) include advanced molecular epidemiology, a novel drug targeting mycolic acid synthesis, and promising vaccine candidates showing high efficacy in animal models. These developments offer new avenues for TB control and treatment.

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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

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Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria

Published on: December 14, 2016

Area of Science:

Mycobacteriology
Infectious Diseases
Epidemiology

Background:

Tuberculosis (TB) remains a significant global health threat, causing millions of deaths annually.
Mycobacterium tuberculosis (M.tb) infects over one-third of the world's population, often as latent infections.
New control strategies are urgently needed to combat the rising incidence and mortality of TB.

Framework:

Molecular epidemiology using 12-locus VNTR analysis for M.tb strain typing.
Identification of Mycobacterium DNA-binding protein 1 (MDP1) as a potential target for anti-TB drugs and vaccines.
Development of serodiagnosis for Mycobacterium avium complex (MAC) pulmonary disease using enzyme immunoassay (EIA).

Implementation:

12-locus VNTR analysis demonstrates superior discriminatory power for M.tb genotyping in Japan compared to IS6110-RFLP.
MDP1, overexpressed in dormant M.tb, plays a crucial role in persistent infection and host protection.
EIA for anti-glycopeptidolipid (GPL) antibodies provides rapid and accurate diagnosis of MAC pulmonary disease.

Implications:

A novel nitroimidazole derivative, OPC-67683, shows potent antimycobacterial activity, including against multidrug-resistant M.tb, and may shorten treatment duration.
Novel DNA vaccine candidates (HVJ-liposome/HSP65 DNA+IL-12 DNA) demonstrate superior protective and therapeutic efficacy in mice and monkeys compared to BCG.
These advancements in diagnostics, therapeutics, and vaccines offer promising new avenues for controlling tuberculosis and MAC infections.