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Updated: Jul 10, 2026

Decellularization and Recellularization Methodology for Human Saphenous Veins
Published on: July 27, 2018
[Influence of human C-type natriuretic peptide on vascular endothelial cell proliferation]
Le Xiao1, Yong-Ming Dang, De Shi
1Department of Vascular Surgery, First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, PR China.
Objective:
To investigate the influence of human C-type natriuretic peptide (hCNP) on proliferation of vascular endothelial cells (HUVECs).
Methods:
Reconstructed pcDNA3.1 (+)/hCNP was transfected into HUVECs with polyethylenimine and its plasmid expression was examined with RT-PCR, immunohistochemistry and Western blot. MTT method was used to determine the effect of expressed protein on proliferation of HUVECs. pcDNA3.1 (+)/hCNP transfection was used for control.
Results:
The proliferation of HUVEC 48 h after pcDNA3.1 (+)/hCNP transfection was (0.301 +/- 0.096), which was obviously higher than that with pcDNA3.1 (+) transfection (0.164 +/- 0.012). Reconstructed pcDNA3.1 (+)/hCNP might be expressed in HUVECs effectively and its protein expression was capable of promoting HUVECs proliferation markedly.
Conclusion:
The successive expression of reconstructed pcDNA3.1 (+)/hCNP and the promoting activity of its expressed protein on HUVECs lay the foundation potential therapeutic value of C-type natriuretic peptide.
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