Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

INTERPOL Review of Forensic Biology and DNA, 2023-2025.

Forensic science international. Synergy·2026

Same author

Lessons from the "Trial of the Century" and early forensic science efforts: Beginnings of U.S. forensic science and the little-known role of the National Bureau of Standards.

Forensic science international·2025

Same author

Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on short tandem repeat sequence nomenclature.

Forensic science international. Genetics·2024

Same author

Special issue: Forensic Genetics: Unde venisti et quo vadis?

Forensic science international. Genetics·2023

Same author

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019-2022.

Forensic science international. Synergy·2023

Same author

Interpol review of forensic biology and forensic DNA typing 2016-2019.

Forensic science international. Synergy·2021

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Short tandem repeat typing technologies used in human identity testing.

John M Butler¹

¹Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA. john.butler@nist.gov

|November 21, 2007

Summary

This summary is machine-generated.

Short tandem repeat (STR) typing is crucial for human identity testing and forensic DNA analysis. This article reviews the technologies and challenges in STR analysis, focusing on PCR amplification and capillary electrophoresis.

More Related Videos

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Published on: April 1, 2019

Related Experiment Videos

Last Updated: Jul 10, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Published on: April 1, 2019

Area of Science:

Forensic Science
Genetics
Molecular Biology

Background:

Short tandem repeat (STR) typing is a cornerstone of modern human identity testing.
Applications span forensic DNA analysis, paternity testing, and disaster victim identification.
Established methods rely on analyzing length-variant STR alleles.

Purpose of the Study:

To provide a concise overview of Short tandem repeat (STR) typing technologies.
To discuss key technical considerations and challenges in current STR analysis.
To highlight the importance of STR typing in forensic and identity applications.

Main Methods:

Multiplex PCR amplification to target multiple STR loci simultaneously.
Capillary electrophoresis (CE) for high-resolution separation of DNA fragments.
Genotyping by comparing electrophoretic profiles to a commercial allelic ladder.

Main Results:

STR typing methods are robust and widely implemented for human identification.
The combination of multiplex PCR and CE offers efficient and accurate STR analysis.
Allelic ladders are essential for reliable interpretation of STR profiles.

Conclusions:

STR typing remains a vital tool in forensic science and human identity testing.
Technological advancements continue to refine STR analysis efficiency and accuracy.
Understanding the underlying technologies and potential issues is key for effective STR typing.