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Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Published on: July 26, 2017
Lysosomotropic agents induce morphological and functional changes in human muscle cells in vitro
P L Baron1, G Meola, E Scarpini
1Istituto di Clinica Neurologica, Centro Dino Ferrari, Università degli Milano, Italy.
European Journal of Basic and Applied Histochemistry
|January 1, 1991
Summary
Lysosomotropic agents like chloroquine can harm differentiating human muscle cells. Studies show these drugs cause vacuolization and disrupt the muscle cell
Area of Science:
- Cell biology
- Muscle cell differentiation
- Lysosome biology
Background:
- Lysosomes are crucial for cellular degradation and homeostasis.
- Understanding lysosome function is key to muscle cell health.
- Disruptions in lysosomal function can lead to cellular damage.
Purpose of the Study:
- To investigate the effects of specific drugs on lysosome distribution in differentiating human muscle cells.
- To assess the impact of lysosomotropic agents on muscle cell structure and function.
Main Methods:
- Human muscle cells were cultured and treated with propranolol, leupeptin, and chloroquine.
- Cytochemical analysis of acid phosphatase and acridine orange staining were used to evaluate lysosomal activity.
- Electron microscopy examined ultrastructural changes in muscle cells.
Main Results:
- Chloroquine treatment led to significant vacuolization and an enlarged lysosomal apparatus.
- Electron microscopy revealed an increase in lysosome-like bodies and disorganization of the contractile apparatus in multinucleated myotubes.
- Propranolol and leupeptin effects were not detailed in the abstract.
Conclusions:
- Lysosomotropic agents, particularly chloroquine, induce detrimental alterations in differentiating human muscle cells.
- These findings suggest potential harm from lysosomotropic agents in muscle tissue.
- Further research is needed to understand the long-term implications for muscle health.
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