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Altered expression of cell cycle regulatory proteins in benign and malignant bone and soft tissue neoplasms

Marilyn M Bui1, Tapan K Bagui, David C Boulware

  • 1Department of Interdisciplinary Oncology, University of South Florida, College of Medicine, Tampa, FL 33612-9497, USA.

In Vivo (Athens, Greece)
|November 21, 2007
PubMed
Abstract

Insights

Cyclin D1 and D3 expression is elevated in bone and soft tissue sarcomas compared to normal tissues and leiomyomas. This suggests cyclins play a role in sarcomagenesis and cell cycle deregulation in these tumors.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Pathology

Background:

  • Cell proliferation is regulated by cyclin binding to cyclin-dependent kinases.
  • Overexpression of cyclins is implicated in deregulating the cell cycle in human tumors.

Purpose of the Study:

  • To assess the expression of G1 cyclins (D1 and D3) and Ki-67 in bone and soft tissue sarcomas.
  • To compare cyclin and Ki-67 expression in sarcomas with adjacent normal tissue and leiomyomas.

Main Methods:

  • Immunohistochemical analysis of cyclin D1, cyclin D3, and Ki-67 on 29 human bone and soft tissue sarcomas.
  • Comparison with adjacent normal tissues and leiomyomas.
  • Western blot analysis of cyclin D3 in human sarcoma cell lines.

Main Results:

  • Cyclin D1 nuclear positivity in 28% of sarcomas, absent in leiomyomas.
  • Cyclin D3 nuclear positivity in 62% of sarcomas, absent in leiomyomas.
  • Ki-67 nuclear staining positive in 86% of sarcomas vs. 16% of leiomyomas.
  • Upregulation of cyclin D1 and D3 observed across various sarcoma types, with differential expression patterns.
  • Normal adjacent tissues were negative for cyclins D1/D3 and showed low Ki-67 expression.

Conclusions:

  • Elevated expression of cyclin D1, D3, and Ki-67 in sarcomas suggests a role in cell cycle deregulation.
  • These findings indicate cyclins may contribute to human sarcomagenesis.
  • Cyclin expression patterns could be relevant for understanding tumor development.

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