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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
Published on: November 13, 2017
Advanced homogeneous-heterogeneous immunosensing format employing restricted access supports
Dolors Jornet1, Miguel Angel Gonzalez-Martínez, Angel Maquieira
1Departamento de Química, Instituto de Química Molecular Aplicada, Universidad Politécnica de Valencia, Camino de Vera s/n, 46071 Valencia, Spain.
Analytical Chemistry
|November 21, 2007
Summary
This study introduces a rapid immunosensing method for detecting atrazine in water. The novel approach achieves quick, automated analysis with a low detection limit, demonstrating its potential for environmental monitoring.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Biotechnology
Background:
- Immunoassays are crucial for detecting environmental contaminants.
- Developing rapid and sensitive detection methods is essential for water quality monitoring.
- Existing methods may lack speed, automation, or sensitivity for real-time analysis.
Purpose of the Study:
- To present a novel, rapid immunosensing methodology using a homogeneous-heterogeneous assay mode.
- To demonstrate the sensor's capability for automated atrazine determination.
- To establish a general labeling methodology for fluorescent tracers in immunosensing.
Main Methods:
- Utilized a homogeneous competition assay involving analyte, fluorescent tracer, and antibody.
- Employed a restricted access alkyl-diol silica C18 reversed-phase chromatographic support for separation.
- Synthesized fluorescent tracers from oligonucleotides with hapten and marker conjugation.
Main Results:
- Achieved automated atrazine determination at a rate of 2 minutes per sample.
- Established a limit of detection of 1.0 microg/L for the optimized system.
- Demonstrated the sensor's capability to analyze natural water samples with tolerance to organic solvents.
Conclusions:
- The developed immunosensor offers a rapid and automated solution for atrazine detection.
- The homogeneous-heterogeneous assay mode combined with chromatographic separation enhances efficiency.
- This methodology shows promise for sensitive and reliable environmental water analysis.
