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Positive Streptobacillus moniliformis PCR in guinea pigs likely due to Leptotrichia spp
Ron Boot1, Lia Van de Berg, Frans A G Reubsaet
1Section of Laboratory Animal Microbiology, Diagnostic Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. r.boot@rivm.nl
Abstract:
Streptobacillus moniliformis is a zoonotic bacterium. We obtained positive S. moniliformis PCR results in oral swab samples from guinea pigs from an experimental colony and the breeding colony of origin. Comparison of the DNA sequence of an amplicon with deposited 16S rDNA sequences revealed that Leptotrichia sp. can be the source of a false positive S. moniliformis PCR outcome.
Insights
A study found that the bacterium Streptobacillus moniliformis, a zoonotic pathogen, was detected in guinea pig oral swabs. However, false positive PCR results can occur due to contamination from Leptotrichia species.
Area of Science:
- Veterinary Microbiology
- Bacteriology
- Zoonotic Diseases
Background:
- Streptobacillus moniliformis is a zoonotic bacterium known to cause infections in various animal species.
- Accurate detection of S. moniliformis is crucial for disease control and public health.
Purpose of the Study:
- To investigate the presence of S. moniliformis in guinea pig colonies.
- To identify potential sources of false positive results in PCR detection.
Main Methods:
- Oral swab samples were collected from guinea pigs in experimental and breeding colonies.
- Polymerase Chain Reaction (PCR) was used to detect S. moniliformis DNA.
- DNA sequencing of PCR amplicons was performed for precise identification.
Main Results:
- Positive S. moniliformis PCR results were obtained from oral swabs of guinea pigs.
- Sequence comparison revealed that Leptotrichia sp. can lead to false positive S. moniliformis PCR outcomes.
- This highlights a potential diagnostic challenge in S. moniliformis detection.
Conclusions:
- Leptotrichia sp. poses a risk of false positive results in S. moniliformis PCR assays.
- Careful interpretation of PCR results and consideration of potential cross-reactivity are essential.
- Further studies may be needed to refine diagnostic methods for S. moniliformis.
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