Sensitive and rapid detection of Aeromonas caviae in stool samples by loop-mediated isothermal amplification
Xiao Na Wei1, Ze Jun Zheng, Li Huan Zhang
1Department of Biochemistry and Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, China.
Abstract:
In this study, a loop-mediated isothermal amplification assay targeting the 16S-23S intergenic spacer regions (internal transcribed spacer) of Aeromonas caviae was developed. Eighteen reference strains and 109 clinical samples were analyzed. The results showed this detection technique is more reliable and convenient compared with common polymerase chain reaction and biochemical culture methods.
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