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Updated: Jul 10, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
Notch signaling in mandibular condylar cartilage development
Takako Shimizu1, K Nakano, H Tsujigiwa
1Hard Tissue Pathology Unit, Matsumoto Dental University Institute for Oral Science, 1780 Hirooka-Gobara, Shiojiri, 399-0781 Japan. tshimizu@po.mdu.ac.jp
Notch signaling is crucial for mandibular condylar cartilage development. Its expression patterns, studied via immunohistochemistry and in situ hybridization in mice, reveal its essential role in cartilage morphogenesis.
Area of Science:
- Developmental biology
- Cell signaling
- Orthodontics
Background:
- Mandibular condylar cartilage is a critical secondary cartilage for jaw development.
- Understanding the molecular mechanisms governing its formation is essential for addressing craniofacial abnormalities.
Purpose of the Study:
- To investigate the spatiotemporal expression of Notch signaling during mandibular condylar cartilage development.
- To elucidate the role of Notch signaling in the morphogenesis of this secondary cartilage.
Main Methods:
- Analysis of ddY mouse mandibular condyles from embryonic day 14 (E14) to postnatal day (E19).
- Histological, immunohistochemical (IHC), and in situ hybridization (ISH) techniques were employed on serial sagittal sections.
Main Results:
- Notch1 intracellular domain (NICD) was detected in nuclei of mesenchymal cells at E14, and in nuclei and cytoplasm from E15 onwards.
- Notch1 mRNA expression was observed in proliferating chondrocytes at E14 and throughout all layers from E15 to E19.
- Mandibular condylar cartilage formation, marked by toluidine blue metachromasia, was evident from E15.
Conclusions:
- The expression patterns of Notch signaling components indicate its involvement in mandibular condylar cartilage development.
- Notch signaling is essential for the morphogenesis and overall development of mandibular condylar cartilage.
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