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Hematopoietic development from human embryonic stem cells.

Mickie Bhatia1

  • 1Mickie Bhatia, PhD, Michael G. DeGroote School of Medicine, McMaster University, 1200 Main St., West, MDCL 5029, Hamilton, ON L8N 3Z5, Canada. mbhatia@mcmaster.ca

Hematology. American Society of Hematology. Education Program
|November 21, 2007
PubMed
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Hematopoietic stem cell (HSC) transplantation is common, but donor cell limitations exist. Human embryonic stem cells (hESCs) offer an alternative source, though their in vivo potential requires further study for clinical use.

Area of Science:

  • Stem cell biology
  • Hematopoiesis
  • Regenerative medicine

Background:

  • Hematopoietic stem cell (HSC) transplantation is a primary cell-based therapy.
  • HSCs possess self-renewal and multilineage differentiation capabilities essential for blood cell production.
  • Current HSC sources (bone marrow, peripheral blood, cord blood) face quantity and compatibility limitations.

Purpose of the Study:

  • To explore human embryonic stem cells (hESCs) as an alternative source for hematopoietic stem cells.
  • To evaluate the potential of hESCs to differentiate into various hematopoietic lineages.
  • To assess the functional capacity of hESC-derived hematopoietic cells compared to somatic HSCs.

Main Methods:

  • Utilized established differentiation protocols to guide hESCs towards hematopoietic fates.

Related Experiment Videos

  • Characterized the resulting hematopoietic cells for lineage commitment and progenitor capacity.
  • Assessed the in vivo repopulating capacity of hESC-derived hematopoietic cells in immunodeficient mouse models.

    • Main Results:

    • hESCs successfully differentiated into erythroid, myeloid, and lymphoid lineages.
    • hESC-derived hematopoietic progenitors exhibited similar clonogenic capacity and primitive phenotypes to somatic HSCs.
    • Transplanted hESC-derived hematopoietic cells demonstrated limited in vivo repopulating potential in mice.

    Conclusions:

    • Human embryonic stem cells can generate hematopoietic cells with progenitor characteristics.
    • Further research and surrogate models are necessary to improve the efficiency and quality of hESC-derived HSCs for clinical applications.
    • hESCs represent a promising, albeit currently limited, alternative for generating hematopoietic stem cells.