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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
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Published on: November 4, 2016

Identification of Streptococcus uberis multilocus sequence types highly associated with mastitis.

Takehiro Tomita1, Brian Meehan, Nalin Wongkattiya

  • 1Department of Biotechnology and Environmental Biology, RMIT University, Bundoora West Campus, Bundoora, Melbourne, Victoria 3083, Australia.

Applied and Environmental Microbiology
|November 21, 2007
PubMed
Summary

Multilocus sequence typing identified specific Streptococcus uberis genotypes linked to mastitis and others to healthy cows. This study also found identical Streptococcus uberis sequence types across continents, indicating global spread.

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Last Updated: Jul 10, 2026

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Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Area of Science:

  • Veterinary microbiology
  • Bacterial genomics
  • Animal health

Background:

  • Streptococcus uberis is a significant cause of bovine mastitis.
  • Understanding the genetic diversity of S. uberis is crucial for developing effective control strategies.

Purpose of the Study:

  • To investigate the genetic diversity of Streptococcus uberis isolates.
  • To correlate specific sequence types with clinical outcomes in dairy cattle.
  • To determine the geographical distribution of S. uberis sequence types.

Main Methods:

  • Multilocus sequence typing (MLST) was employed to analyze S. uberis isolates.
  • Statistical analysis was used to associate genotypes with mastitis status and somatic cell counts.
  • Phylogenetic analysis was performed to assess population structure and recombination.

Main Results:

  • Two distinct clusters of S. uberis isolates were identified: one associated with clinical/subclinical mastitis and another with low somatic cell counts.
  • Global clonal complex (GCC) sequence types (ST) 5s and ST143s were significantly associated with mastitis (P = 0.006), suggesting virulent lineages.
  • GCC ST86 was associated with isolates from cows with low somatic cell counts.
  • Identical sequence types (ST60 and ST184) were found in isolates from Australasia and Europe, demonstrating intercontinental distribution.
  • Analysis indicated substantial recombination within the Australian S. uberis population.

Conclusions:

  • Specific S. uberis genotypes are linked to virulence and mastitis development.
  • Certain sequence types are associated with commensal or less pathogenic S. uberis strains.
  • The global distribution of identical sequence types highlights the need for international surveillance.
  • Significant recombination occurs within S. uberis populations, influencing its evolution.