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Related Experiment Videos

Quality control limits for disk diffusion and broth microdilution susceptibility tests with Haemophilus test medium.

G V Doern1, E H Gerlach, J H Jorgensen

  • 1Department of Clinical Microbiology, University of Massachusetts Medical Center, Worcester 01655.

Diagnostic Microbiology and Infectious Disease
|November 11, 1991
PubMed
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This study established quality control ranges for antimicrobial susceptibility testing of Haemophilus influenzae. These findings ensure reliable laboratory results for guiding patient treatment decisions.

Area of Science:

  • Clinical Microbiology
  • Antimicrobial Resistance
  • Bacterial Pathogenesis

Background:

  • Accurate antimicrobial susceptibility testing (AST) is crucial for effective treatment of Haemophilus influenzae infections.
  • Standardized quality control (QC) parameters are essential for ensuring the reliability of AST methods.
  • Haemophilus influenzae remains a significant pathogen, necessitating robust diagnostic capabilities.

Purpose of the Study:

  • To develop and propose quality control test limits for disk diffusion and broth microdilution susceptibility testing.
  • To establish QC ranges for Haemophilus influenzae ATCC 49247 using Haemophilus test medium.
  • To support accurate and reproducible AST for a panel of 18 key antimicrobial agents.

Main Methods:

  • A collaborative study involving six clinical laboratories.

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  • Utilized Haemophilus influenzae ATCC 49247 as the reference strain.
  • Performed both disk diffusion and broth microdilution susceptibility testing methods.
  • Tested 18 different antimicrobial agents against the reference strain.

    • Main Results:

    • Established specific quality control ranges for disk diffusion and broth microdilution methods.
    • Provided QC ranges for ampicillin, amoxicillin-clavulanate, cefotaxime, ceftriaxone, trimethoprim-sulfamethoxazole, ciprofloxacin, and 12 other antimicrobials.
    • Demonstrated inter-laboratory reproducibility of the proposed QC ranges.

    Conclusions:

    • The study successfully developed and validated quality control limits for H. influenzae AST.
    • The proposed QC ranges will enhance the accuracy and reliability of susceptibility testing in clinical laboratories.
    • These standardized QC parameters are vital for monitoring antimicrobial resistance trends and optimizing patient therapy.