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Related Experiment Video

Updated: Jul 10, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

Solid-state fermentation for enhanced production of laccase using indigenously isolated Ganoderma sp.

Madhavi S Revankar¹, Kiran M Desai, S S Lele

¹Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400019, India.

Applied Biochemistry and Biotechnology

|November 21, 2007

Summary

Researchers optimized laccase production using solid-state fermentation (SSF) with wheat bran and Ganoderma sp. This method achieved high laccase yields, offering a sustainable way to utilize agricultural waste.

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Area of Science:

Biotechnology
Enzyme Production
Microbial Fermentation

Background:

Laccases are versatile enzymes with numerous industrial applications.
Solid-state fermentation (SSF) offers an eco-friendly approach for enzyme production using agro-residues.
Ganoderma sp. is a white rot fungus known for its potential in lignin degradation and enzyme synthesis.

Purpose of the Study:

To optimize laccase production by an indigenous Ganoderma sp. isolate using SSF.
To identify the optimal agricultural waste substrate and fermentation conditions for maximizing laccase yield.
To enhance laccase production through medium supplementation and advanced optimization techniques.

Main Methods:

Screening of various agricultural wastes for laccase production.
Optimization of SSF parameters (substrate, moisture, inoculum) using the one-factor-at-a-time method.
Medium supplementation with carbon (starch) and nitrogen (yeast extract) sources.
Response surface methodology (RSM) for further optimization of enhanced medium.

Main Results:

Wheat bran was identified as the most effective substrate for laccase production.
Optimal SSF conditions yielded 2,400 U/g dry substrate (U/gds) of laccase.
Supplementation and RSM optimization led to a fourfold increase in laccase activity, reaching 10,050 U/gds.
The indigenous Ganoderma sp. isolate demonstrated significant laccase-producing potential.

Conclusions:

The optimized SSF process using indigenous Ganoderma sp. is highly effective for laccase production.
This method provides a cost-effective strategy for valorizing agricultural waste into valuable enzymes.
The study highlights the potential of SSF for sustainable and large-scale enzyme manufacturing.