Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles.

Nature protocols·2026

Same author

Multi-fluid, multi-omics signatures of insulin resistance and incident type 2 diabetes among Puerto Rican adults.

Frontiers in endocrinology·2025

Same author

Differentially Expressed Salivary miRNAs in Temporomandibular Disorders.

Orthodontics & craniofacial research·2025

Same author

Saliva liquid biopsy for detection of early-stage lesions.

Expert review of molecular diagnostics·2025

Same author

Cell-Free DNA: Features and Attributes Shaping the Next Frontier in Liquid Biopsy.

Molecular diagnosis & therapy·2025

Same author

Rapid and comprehensive detection of viral antibodies and nucleic acids via an acoustofluidic integrated molecular diagnostics chip: AIMDx.

Science advances·2025

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Published on: April 1, 2019

Single nucleotide polymorphism mapping array assay.

Xiaofeng Zhou, David T W Wong

Methods in Molecular Biology (Clifton, N.J.)

|November 21, 2007

Summary

Single nucleotide polymorphisms (SNPs) offer advantages for DNA analysis due to their abundance and stability. This study details a protocol for high-throughput whole genome SNP genotyping using Affymetrix arrays.

Area of Science:

Genetics
Genomics
Molecular Biology

Background:

Single nucleotide polymorphisms (SNPs) are the most common type of DNA variation in the human genome.
SNPs offer advantages over other genetic markers due to their abundance, even spacing, and stability.
Millions of SNPs have been identified, enabling large-scale genomic studies.

Purpose of the Study:

To provide a detailed experimental protocol for whole genome SNP genotyping.
To demonstrate the application of Affymetrix GeneChip SNP Mapping Arrays for high-throughput SNP analysis.

Main Methods:

Utilizing high-density oligonucleotide arrays for SNP analysis.
Implementing the Affymetrix GeneChip SNP Mapping Array for whole genome SNP genotyping.
Automating SNP scoring for large-scale analysis.

More Related Videos

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Related Experiment Videos

Last Updated: Jul 10, 2026

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Published on: April 1, 2019

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Main Results:

High-density SNP arrays enable genotyping of hundreds of thousands of SNP markers.
The Affymetrix GeneChip SNP Mapping Array facilitates efficient whole genome SNP analysis.
Automated SNP scoring supports high-throughput applications.

Conclusions:

SNP genotyping assays using high-density arrays are powerful tools for genomic research.
The described protocol enables comprehensive whole genome SNP analysis.
Advancements in SNP array technology significantly improve the scale and efficiency of genetic studies.