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Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Changes in the Appendicular Skeleton with Age

Changes in the Appendicular Skeleton with Age

The upper and lower limb initially develops as a small bulge called a limb bud, which appears on the lateral side of the early embryo. The upper limb bud appears near the end of the fourth week of development, with the lower limb bud appearing shortly after.
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...

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Related Experiment Video

Updated: Jul 10, 2026

Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

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Published on: November 17, 2016

Application of sonic hedgehog to the developing chick limb.

Eva Tiecke, Cheryll Tickle

Methods in Molecular Biology (Clifton, N.J.)

|November 21, 2007

Summary

This study details methods for applying Sonic hedgehog (Shh) to chick limb development. Shh signaling controls digit formation and pattern, crucial for vertebrate embryogenesis.

Area of Science:

Developmental Biology
Embryology
Molecular Biology

Background:

Sonic hedgehog (Shh) is a key signaling molecule in vertebrate embryonic development.
Shh is expressed in the polarizing region of the limb bud, influencing digit patterning.
Understanding Shh signaling is vital for studying limb development and congenital abnormalities.

Purpose of the Study:

To describe methods for applying Sonic hedgehog (Shh) to developing chick limbs.
To investigate the role of Shh in controlling digit number and pattern.
To explore Shh's effects on digit morphogenesis at later developmental stages.

Main Methods:

Application of Shh-expressing cells to early chick limb buds.
Direct application of Shh protein to limb bud regions.

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Observation of effects on digit formation and morphogenesis.

Main Results:

Application of Shh mimics endogenous polarizing region signaling.
Shh application influences digit number and pattern in developing chick limbs.
Shh protein application can alter digit morphogenesis in later developmental stages.

Conclusions:

Methods for Shh application provide a tool to study limb development.
Shh signaling is pivotal in establishing digit identity and arrangement.
Shh plays a significant role in both early patterning and later morphogenesis of chick limbs.