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Updated: Jul 10, 2026
Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents
Published on: June 2, 2015
Ischemic preconditioning protects the dog kidney from ischemia-reperfusion injury
Mehdi Salehipour1, Abdolaziz Khezri, Ahmad Monabbati
1Division of Urology, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran. E-Mail salehipour@sums.ac.ir
Objective:
To evaluate the effects of unilateral ischemic insult and ischemic preconditioning (IPC) on renal histology in a canine model.
Methods:
30 dogs were randomized into 4 groups. In group A (5 male controls) and group B (5 female controls), ischemia was induced by clamping both left renal arteries for 40 min. Dogs in group C (10 male cases) or group D (10 female cases) underwent 5 min of arterial clamping and 10 min of declamping prior to the final 40-min ischemia induction. Renal biopsy was prepared 48 h later and microscopically examined.
Results:
The control groups (A and B) developed 40% frank necrosis, 60% moderate injury, and there was no intact renal tissue in this group with no difference between sexes. The IPC groups (C and D) revealed 55% moderate injury and 45% normal pathology; however, there was no frank necrosis among them. Better IPC protection in the female group was not statistically significant.
Conclusion:
An IPC schedule of 5-min ischemia and 10-min reperfusion improves ischemia-reperfusion injury from subsequent prolonged ischemia in a canine model.
