Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ESCRT-0 regulates AMPA receptor currents and Ca<sup>2+</sup>- dependent signaling.

bioRxiv : the preprint server for biology·2026
Same author

PrP<sup>C</sup>-facilitated cell signaling activates phospholipase Cɣ1 and triggers an Arc/Arg3.1 response in mouse and iPSC-derived human neurons.

Stem cell reports·2026
Same author

Gpnmb defines a phagocytic state of microglia linked to cell death in prion disease mouse model.

Nature communications·2026
Same author

Altered crosstalk of bacterial lipopolysaccharide with immune cells in colorectal cancer compared to paired adjacent intestinal tissue.

Gut microbes·2026
Same author

Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex.

PLoS pathogens·2026
Same author

Creutzfeldt-Jakob disease is an Alzheimer's disease-related dementia.

Trends in molecular medicine·2026

Related Experiment Video

Updated: Jul 10, 2026

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

Prion strain discrimination using luminescent conjugated polymers.

Christina J Sigurdson1, K Peter R Nilsson, Simone Hornemann

  • 1UniversitätsSpital Zürich, Institute of Neuropathology, Department of Pathology, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland.

Nature Methods
|November 21, 2007
PubMed
Summary

Luminescent conjugated polymers (LCPs) can distinguish between different prion strains by analyzing their unique fluorescence spectra. This method aids in identifying structural variations in prion protein aggregates linked to disease phenotypes.

More Related Videos

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Related Experiment Videos

Last Updated: Jul 10, 2026

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Area of Science:

  • Neuroscience
  • Biochemistry
  • Materials Science

Background:

  • Multiple prion strains suggest conformational diversity in disease-associated PrP(Sc) aggregates, distinct from cellular PrP(C).
  • Characterizing these distinct prion strains is crucial for understanding and diagnosing prion-related diseases.

Purpose of the Study:

  • To utilize luminescent conjugated polymers (LCPs) for characterizing and differentiating prion strains based on their unique structural conformations.
  • To explore the potential of LCPs in identifying subtle structural differences in prion protein aggregates.

Main Methods:

  • Employed luminescent conjugated polymers (LCPs) that exhibit conformation-dependent fluorescence spectra.
  • Analyzed LCP reactivity and emission spectra from brain sections of various prion-infected animals.
  • Generated fibrils from recombinant PrP to investigate morphology-dependent spectral characteristics.

Main Results:

  • LCPs successfully discriminated among four immunohistochemically indistinguishable prion strains (scrapie, CWD, BSE, RML scrapie).
  • Differentiated between field isolates of BSE and bovine amyloidotic spongiform encephalopathy.
  • Identified noncongophilic prion deposits in deer and sheep.
  • Observed unique LCP spectra from recombinant PrP fibrils with distinct morphologies, indicating supramolecular structure variations.

Conclusions:

  • LCPs offer a sensitive method for characterizing prion strains based on structural differences.
  • This technique can identify distinct protein aggregate structures and link them to specific disease phenotypes.
  • LCPs show promise for detecting and differentiating protein conformational variations in neurodegenerative diseases.