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Problems in interpretation of piperacillin susceptibility of TEM-1 producing Escherichia coli in the disk diffusion
T Arstila1, S Huovinen, P Huovinen
1Department of Medical Microbiology, Turku University, Finland.
Abstract:
The susceptibility of 455 Escherichia coli blood culture isolate to piperacillin was tested with the disk diffusion test. The presence of different beta-lactamase genes in these strains was also studied using DNA hybridization. Of the TEM beta-lactamase producing isolates, 64% (61/95) were interpreted as intermediately susceptible to piperacillin. Because piperacillin is hydrolyzed by TEM-type beta-lactamases, we suggest that the intermediate susceptibility category should be reduced or omitted in testing piperacillin susceptibility of Escherichia coli isolates.
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